Potential therapeutic effects of autologous platelet rich plasma on impaired wound healing: a prospective clinical study


  • Sachin Upadhyay Department of Orthopaedics, Traumatology and Rehabilitation, Netaji Subhash Chandra Bose Medical College, Jabalpur, Madhya Pradesh, India
  • Hashmukh Shantilal Varma Department of Orthopaedics, Traumatology and Rehabilitation, Netaji Subhash Chandra Bose Medical College, Jabalpur, Madhya Pradesh, India
  • Sumit Yadav Department of Orthopaedics, Traumatology and Rehabilitation, Netaji Subhash Chandra Bose Medical College, Jabalpur, Madhya Pradesh, India




Autologous platelet rich plasma, Wound healing, Non-healing wound


Background: The evolving state of technology in field of medicine has led to a plethora of new options in the realm of wound healing. The primary objective of present study was to assess the potential therapeutic effects of autologous platelet rich plasma in the healing of wound.

Methods: A prospective clinical nonrandomized cohort study was performed in 50 patients (40 males, 10 females) with recalcitrant, chronic; non-healing wounds of different etiologies. Age ranged from 25 to 70 years (mean 39.14±13.38). Eligible patients were treated with autologous platelet rich plasma (PRP) at 4-days intervals for a maximum of 14 sessions. Patients were evaluated (reduction in size of the wound (area)) every week and continued until final healing has achieved. The primary outcome was positive response to therapy, defined as complete healing of the wound and absence of any raw area. Significance was set at p<0.05.

Results: The mean time duration to wound healing was 6.33 weeks (S.D±2.16). A statistically significant difference (p<0.05) was observed between the baseline parameters and mean area after PRP injection. After 7 sessions (28 days of therapy) the wounds showed the significant change in wound surface area (p<0.05). None of the patients developed deep or superficial wound infections.

Conclusions: There is considerable improvement in wound characteristics like healthy granulation tissue, healing edge, pain, slough, bleeding on touch and discharge after 7 days of PRP therapy and considerable improvement on follow up.


Mast BA. The skin. In: Cohen IK, Diegelmann RF, Lindblad WJ, editors. Wound Healing, Biochemical and Clinical Aspects. Philadelphia, PA: W.B. Saunders; 1992.

Snyder RJ Treatment of nonhealing ulcers with allografts. Clin Dermatol. 2005;23(4):388-95.

Augustin M, Maier K. Psychosomatic Aspects of Chronic Wounds. Dermatol Psychosomatics. 2003;4:5–13.

Mustoe T. Understanding chronic wounds:a unifying hypothesis on their pathogenesis and implications for therapy. Am J Surg. 2004;187(5):65S-70S.

Bhat S. Srb’s Manual of Surgery. 4th edition. Jaypee Brothers Medical Pub; 2013: 17.

Martinez-Zapata MJ, Martí-Carvajal AJ, Solà I, Expósito JA, Bolíbar I, Rodríguez L, Garcia J.Autologous platelet-rich plasma for treating chronic wounds. Cochrane Database Syst Rev. 2012;10:CD006899.

Sood A, Granick MS, Tomaselli NL. Wound Dressings and Comparative Effectiveness Data. Advances in Wound Care. 2014;3(8):511–29.

Greer N, Foman NA, MacDonald R, Dorrian J, Fitzgerald P, Rutks I, et al. Advanced wound care therapies for non-healing diabetic, venous, and arterial ulcers: a systematic review. 2012;159(8):532-42.

Aminian B, Shams M, Karim-Aghaee B, Soveyd M, Omrani GR. The role of the autologous platelet-derived growth factor in the management of decubitus ulcer. Arch Iranian Med. 1999;2:98–101.

Damir A. Recent advances in management of chronic non-healing diabetic foot ulcers. JIMSA. 2011;24(4):219–23.

Driver R, Hanft J, Fylling P, Beriou JM; Autologel Diabetic Foot Ulcer Study Group. A prospective, randomized, controlled trial of autologous platelet rich plasma for the treatment of diabetic foot ulcers. Ostomy Wound Manage. 2006;52(6):68–87.

Andia I, Abate M. Platelet-rich plasma:underlying biology and clinical correlates. Regen Med. 2013;8(5):645–58.

Suryanarayan S, Budamakuntla L, Khadri SIS, et al. Efficacy of autologous platelet-rich plasma in the treatment of chronic non-healing leg ulcers. Plast Aesthet Res. 2015;1(2):65–9.

Sebastian KMS, Lobato I, Hernandez I, Burgos-Alonso N, Gomez-Fernandez MC, López JL, et al. Efficacy and safety of autologous platelet rich plasma for the treatment of vascular ulcers in primary care: phase III study. BMC Fam Pract. 2014;15:211.

Vaishnavi C, Mohan B, Narayanan LL. Treatment of endodontically induced periapical lesions using hydroxyapatite, platelet-rich plasma, and a combination of both:an in vivo study. J Conserv Dent. 2011;14:140‑6.

Sarvajnamurthy S, Suryanarayan S, Budamakuntala L, Suresh DH. Autologous platelet rich plasma in chronic venous ulcers: study of 17 cases. J Cutan Aesthet Surg. 2013;6:97-9.

Margolis DJ, Kantor J, Berlin JA. Healing of diabetic neuropathic foot ulcers receiving standard treatment. A meta-analysis. Diabetes Care. 1999;22:692–5.

Blume PA, Walters J, Payne W, Ayala J, Lantis J. Comparison of negative pressure wound therapy using vacuum-assisted closure with advanced moist wound therapy in the treatment of diabetic foot ulcers: A multicenter randomized controlled trial. Diabetes Care. 2008;31:631–6.

Knighton DR, Ciresi K, Fiegel VD, Schumerth S, Butler E, Cerra F. Stimulation of repair in chronic, non-healing cutaneous ulcers using platelet derived wound healing formula. Surg Gynecol Obstet. 1990;170(1):56-60.

Frykberg RG, Driver VR, Carmen D. Chronic wounds treated with a physiologically relevant concentration of Platelet rich plasma gel: A prospective case series. Ostomy Wound Management. 2010;56(6):36-4.

Steenvoorde P, Vandoorn LP, Naves C, Oskam J. Use of platelet rich fibrin on hard to heal wounds. J Wound Care. 2008;17(2):60-3.

Kakudo N, Kushida S, Minakata T, Suzuki K, Kusumoto K. Platelet-rich plasma promotes epithelialization and angiogenesis in a splitthickness skin graft donor site. Med Mol Morphol. 2011;44:233‑6.

Crovetti G, Martenelli G, ISSI M, Barone Guizzardi M, Campanati B, et al. Platelet gel for healing cutaneous chronic wounds. Transsfus Apher. 2004;30(2):145-51.

Avishai E, Yeghiazaryan K, Golubnitschaja O. Impaired wound healing: facts and hypotheses for multi-professional considerations in predictive, preventive and personalised medicine. EPMA J. 2017;8(1):23-33.

Marx RE. Platelet-rich plasma:evidence to support its use. J Oral Maxillofac Surg. 2004;62(4):489-96.

Bhanot S, Alex JC. Current applications of platelet gels in facial plastic surgery. Facial Plast Surg. 2002;18(1):27-33.

Silverstein P. Smoking and wound healing. Am J Med. 1992;93(1):22-4.






Original Research Articles